39 4d5 c (Developmental Studies Hybridoma Bank)

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Developmental Studies Hybridoma Bank 39 4d5 c
39 4d5 C, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/39+4d5+c/anti-Islet-1+%26+Islet-2+homeobox/pmc12348247-3-6-4
Average 96 stars, based on 296 article reviews

39 4d5 c - by Bioz Stars, 2026-10

96/100 stars

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Article Title: Using a neonatal rat system as a bioincubator to generate adult-like mature cardiomyocytes from human and mouse pluripotent stem cells
Article Snippet: GlutaMAX (100 x) (Gibco, Cat no: 35050-061) Saponin (Sigma, Cat no: S4521) Mouse anti-Islet1 (Developmental Studies Hybridoma Bank, Iowa City, IA) Donkey anti-mouse IgG secondary antibody, Alexa Fluor 647 conjugate (Thermo Fisher Scientific, Cat no: A-31571, Lot #1757130) Sodium Chloride (Sigma, Cat no: S9888) Potassium Chloride (Sigma, Cat no: P9333) Magnesium Sulfate (Sigma, Cat no: M7506) Sodium Phosphate Monobasic (Sigma, Cat no: S3139) Sodium Bicarbonate (Sigma, Cat no: S5761) Glucose (Sigma, Cat no: D9434) HEPES (Sigma, Cat no: H3375) Magnesium Chloride (Sigma, Cat no: M8266) Calcium Chloride (Sigma, Cat no: C1016) Collagenase type II (Worthington Biochemical Corporation, Cat no: LS004176 ) Protease type XIV (Sigma, Cat no: P5147) Fura-2AM (Thermo Fisher Scientific, Cat no: F1221) Fluor 594-conjugated WGA antibody (Thermo Fisher Scientific, Cat no: W11262 ) ProLong Diamond Antifade mount. (Thermo Fisher Scientific, Cat no: P36961 ) RNase inhibitor (Thermo Fisher Scientific, Cat no: N8080119) DNase I (RNase free) (New England Biolabs, Cat no: M0303S) RNase free water (Qiagen, Cat no: 129112) SMARTscribe reverse transcriptase (Clontech, Cat no: 639536) dNTP Mix (10mM each) (Thermo Fisher Scientific, Cat no: R0191) DTT, 1M (Thermo Fisher Scientific, Cat no: P2325) Advantage 2 Polymerase mix (Clontech, Cat no: 639201) Custom-designed PCR primers (Integrated DNA Technologies) AMPure XP PCR purification kit (Beckman Coulter, Cat no: A63880 ) RPMI 1640 without Glucose (Thermo Fisher Scientific, Cat no 11879020) Sodium L-Lactate (Sigma, Cat no: 71718) Mouse anti-Isl1 antibody, clone 39.4D5-c (Developmental Studies Hybridoma Bank) !

Article Title: Derivation of Peripheral Nociceptive, Mechanoreceptive, and Proprioceptive Sensory Neurons from the same Culture of Human Pluripotent Stem Cells
Article Snippet: AP2a Abcam #ab108311 1:500 ASCL1/MASH1 Bd Pharmingen #556604 1:100 αSMA Sigma #A5528 1:1000 Brn3a Chemicon #MAB1585 1:100 CGRP Neuromics #RA242112 1:100 Isl1 DSHB #39.4D5-c 1:200 Peripherin Santa Cruz #SC-377093/H0112 1:200 Ret1 Sigma #SAB1409600 1:100 Runx1 Sigma #HPA004176 1:75 Sox10 Santa Cruz #sc-365692 and #sc17342 1:100 Substance P Neuromics #GP14103 1:100 TBR1 Millipore #AB10554 1:1000 TUJ1 Biolegend #802001 and #801201 1:1500 Alexa Fluor 488 donkey α mouse invitrogen #A21202 1:400 Alexa Fluor 488 donkey α goat invitrogen #A11055 1:400 Alexa Fluor 647 donkey α rabbit invitrogen #A31573 1:400 Alexa Fluor 647 donkey α mouse invitrogen #A31571 1:400 Alexa Fluor 488 donkey α rabbit invitrogen #A21206 1:400 Alex Fluor 488 goat α mouse IgG1 invitrogen #A21121 1:400 Alexa Fluor 647 goat α mouse IgG2 invitrogen #A21241 1:400 CD49d-PE/Cy7 Biolegend #304314 1:20 TRKA-PE R&D #FAB1751P 1:20 TRKB-AF647 R&D #FAB3971R 1:20 TRKC-PE R&D #FAB373P 1:20 anti-mouse IgG Jackson ImmunoResearch #115-005-003 1:325 anti-goat IgG Jackson ImmunoResearch #705-005-003 1:325 TRKA R&D MAB1751R 1:100 TRKB R&D MAB3971 1:100 TRKC R&D AF373 1:100

Article Title: Regenerating Corticospinal Axons Innervate Phenotypically Appropriate Neurons within Neural Stem Cell Grafts
Article Snippet: Mouse Islet 1/2 , DSHB , Cat# 39.4D5-c.

Article Title: Optimized Protocol to Generate Spinal Motor Neuron Cells from Induced Pluripotent Stem Cells from Charcot Marie Tooth Patients
Article Snippet: Islet1/2 , DSHB , 39.4D5-c , Mouse Mono IgG , 1:25.

Article Title: Protocol for the induction of human spinal motor neurons from human induced pluripotent stem cells for studying amyotrophic lateral sclerosis
Article Snippet: Anti-isl1, dilution 1:1,000 , DSHB , 39.4D5-C.

Whole Genome Amplification:

Article Title: Using a neonatal rat system as a bioincubator to generate adult-like mature cardiomyocytes from human and mouse pluripotent stem cells
Article Snippet: .. GlutaMAX (100 x) (Gibco, Cat no: 35050-061) Saponin (Sigma, Cat no: S4521) Mouse anti-Islet1 (Developmental Studies Hybridoma Bank, Iowa City, IA) Donkey anti-mouse IgG secondary antibody, Alexa Fluor® 647 conjugate (Thermo Fisher Scientific, Cat no: A-31571, Lot #1757130) Sodium Chloride (Sigma, Cat no: S9888) Potassium Chloride (Sigma, Cat no: P9333) Magnesium Sulfate (Sigma, Cat no: M7506) Sodium Phosphate Monobasic (Sigma, Cat no: S3139) Sodium Bicarbonate (Sigma, Cat no: S5761) Glucose (Sigma, Cat no: D9434) HEPES (Sigma, Cat no: H3375) Magnesium Chloride (Sigma, Cat no: M8266) Calcium Chloride (Sigma, Cat no: C1016) Collagenase type II (Worthington Biochemical Corporation, Cat no: LS004176 ) Protease type XIV (Sigma, Cat no: P5147) Fura-2AM (Thermo Fisher Scientific, Cat no: F1221) Fluor 594-conjugated WGA antibody (Thermo Fisher Scientific, Cat no: W11262 ) ProLong® Diamond Antifade mount. (Thermo Fisher Scientific, Cat no: P36961 ) RNase inhibitor (Thermo Fisher Scientific, Cat no: N8080119) DNase I (RNase free) (New England Biolabs, Cat no: M0303S) RNase free water (Qiagen, Cat no: 129112) SMARTscribe reverse transcriptase (Clontech, Cat no: 639536) dNTP Mix (10mM each) (Thermo Fisher Scientific, Cat no: R0191) DTT, 1M (Thermo Fisher Scientific, Cat no: P2325) Advantage ® 2 Polymerase mix (Clontech, Cat no: 639201) Custom-designed PCR primers (Integrated DNA Technologies) AMPure XP PCR purification kit (Beckman Coulter, Cat no: A63880 ) RPMI 1640 without Glucose (Thermo Fisher Scientific, Cat no 11879020) Sodium L-Lactate (Sigma, Cat no: 71718) Mouse anti-Isl1 antibody, clone 39.4D5-c (Developmental Studies Hybridoma Bank) ! ..

Reverse Transcription:

Article Title: Using a neonatal rat system as a bioincubator to generate adult-like mature cardiomyocytes from human and mouse pluripotent stem cells
Article Snippet: .. GlutaMAX (100 x) (Gibco, Cat no: 35050-061) Saponin (Sigma, Cat no: S4521) Mouse anti-Islet1 (Developmental Studies Hybridoma Bank, Iowa City, IA) Donkey anti-mouse IgG secondary antibody, Alexa Fluor® 647 conjugate (Thermo Fisher Scientific, Cat no: A-31571, Lot #1757130) Sodium Chloride (Sigma, Cat no: S9888) Potassium Chloride (Sigma, Cat no: P9333) Magnesium Sulfate (Sigma, Cat no: M7506) Sodium Phosphate Monobasic (Sigma, Cat no: S3139) Sodium Bicarbonate (Sigma, Cat no: S5761) Glucose (Sigma, Cat no: D9434) HEPES (Sigma, Cat no: H3375) Magnesium Chloride (Sigma, Cat no: M8266) Calcium Chloride (Sigma, Cat no: C1016) Collagenase type II (Worthington Biochemical Corporation, Cat no: LS004176 ) Protease type XIV (Sigma, Cat no: P5147) Fura-2AM (Thermo Fisher Scientific, Cat no: F1221) Fluor 594-conjugated WGA antibody (Thermo Fisher Scientific, Cat no: W11262 ) ProLong® Diamond Antifade mount. (Thermo Fisher Scientific, Cat no: P36961 ) RNase inhibitor (Thermo Fisher Scientific, Cat no: N8080119) DNase I (RNase free) (New England Biolabs, Cat no: M0303S) RNase free water (Qiagen, Cat no: 129112) SMARTscribe reverse transcriptase (Clontech, Cat no: 639536) dNTP Mix (10mM each) (Thermo Fisher Scientific, Cat no: R0191) DTT, 1M (Thermo Fisher Scientific, Cat no: P2325) Advantage ® 2 Polymerase mix (Clontech, Cat no: 639201) Custom-designed PCR primers (Integrated DNA Technologies) AMPure XP PCR purification kit (Beckman Coulter, Cat no: A63880 ) RPMI 1640 without Glucose (Thermo Fisher Scientific, Cat no 11879020) Sodium L-Lactate (Sigma, Cat no: 71718) Mouse anti-Isl1 antibody, clone 39.4D5-c (Developmental Studies Hybridoma Bank) ! ..

Polymerase Chain Reaction:

Article Title: Using a neonatal rat system as a bioincubator to generate adult-like mature cardiomyocytes from human and mouse pluripotent stem cells
Article Snippet: .. GlutaMAX (100 x) (Gibco, Cat no: 35050-061) Saponin (Sigma, Cat no: S4521) Mouse anti-Islet1 (Developmental Studies Hybridoma Bank, Iowa City, IA) Donkey anti-mouse IgG secondary antibody, Alexa Fluor® 647 conjugate (Thermo Fisher Scientific, Cat no: A-31571, Lot #1757130) Sodium Chloride (Sigma, Cat no: S9888) Potassium Chloride (Sigma, Cat no: P9333) Magnesium Sulfate (Sigma, Cat no: M7506) Sodium Phosphate Monobasic (Sigma, Cat no: S3139) Sodium Bicarbonate (Sigma, Cat no: S5761) Glucose (Sigma, Cat no: D9434) HEPES (Sigma, Cat no: H3375) Magnesium Chloride (Sigma, Cat no: M8266) Calcium Chloride (Sigma, Cat no: C1016) Collagenase type II (Worthington Biochemical Corporation, Cat no: LS004176 ) Protease type XIV (Sigma, Cat no: P5147) Fura-2AM (Thermo Fisher Scientific, Cat no: F1221) Fluor 594-conjugated WGA antibody (Thermo Fisher Scientific, Cat no: W11262 ) ProLong® Diamond Antifade mount. (Thermo Fisher Scientific, Cat no: P36961 ) RNase inhibitor (Thermo Fisher Scientific, Cat no: N8080119) DNase I (RNase free) (New England Biolabs, Cat no: M0303S) RNase free water (Qiagen, Cat no: 129112) SMARTscribe reverse transcriptase (Clontech, Cat no: 639536) dNTP Mix (10mM each) (Thermo Fisher Scientific, Cat no: R0191) DTT, 1M (Thermo Fisher Scientific, Cat no: P2325) Advantage ® 2 Polymerase mix (Clontech, Cat no: 639201) Custom-designed PCR primers (Integrated DNA Technologies) AMPure XP PCR purification kit (Beckman Coulter, Cat no: A63880 ) RPMI 1640 without Glucose (Thermo Fisher Scientific, Cat no 11879020) Sodium L-Lactate (Sigma, Cat no: 71718) Mouse anti-Isl1 antibody, clone 39.4D5-c (Developmental Studies Hybridoma Bank) ! ..

Purification:

Article Title: Using a neonatal rat system as a bioincubator to generate adult-like mature cardiomyocytes from human and mouse pluripotent stem cells
Article Snippet: .. GlutaMAX (100 x) (Gibco, Cat no: 35050-061) Saponin (Sigma, Cat no: S4521) Mouse anti-Islet1 (Developmental Studies Hybridoma Bank, Iowa City, IA) Donkey anti-mouse IgG secondary antibody, Alexa Fluor® 647 conjugate (Thermo Fisher Scientific, Cat no: A-31571, Lot #1757130) Sodium Chloride (Sigma, Cat no: S9888) Potassium Chloride (Sigma, Cat no: P9333) Magnesium Sulfate (Sigma, Cat no: M7506) Sodium Phosphate Monobasic (Sigma, Cat no: S3139) Sodium Bicarbonate (Sigma, Cat no: S5761) Glucose (Sigma, Cat no: D9434) HEPES (Sigma, Cat no: H3375) Magnesium Chloride (Sigma, Cat no: M8266) Calcium Chloride (Sigma, Cat no: C1016) Collagenase type II (Worthington Biochemical Corporation, Cat no: LS004176 ) Protease type XIV (Sigma, Cat no: P5147) Fura-2AM (Thermo Fisher Scientific, Cat no: F1221) Fluor 594-conjugated WGA antibody (Thermo Fisher Scientific, Cat no: W11262 ) ProLong® Diamond Antifade mount. (Thermo Fisher Scientific, Cat no: P36961 ) RNase inhibitor (Thermo Fisher Scientific, Cat no: N8080119) DNase I (RNase free) (New England Biolabs, Cat no: M0303S) RNase free water (Qiagen, Cat no: 129112) SMARTscribe reverse transcriptase (Clontech, Cat no: 639536) dNTP Mix (10mM each) (Thermo Fisher Scientific, Cat no: R0191) DTT, 1M (Thermo Fisher Scientific, Cat no: P2325) Advantage ® 2 Polymerase mix (Clontech, Cat no: 639201) Custom-designed PCR primers (Integrated DNA Technologies) AMPure XP PCR purification kit (Beckman Coulter, Cat no: A63880 ) RPMI 1640 without Glucose (Thermo Fisher Scientific, Cat no 11879020) Sodium L-Lactate (Sigma, Cat no: 71718) Mouse anti-Isl1 antibody, clone 39.4D5-c (Developmental Studies Hybridoma Bank) ! ..

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